Assessing the influence of mechanical ventilation on blood gases and blood pressure in rattlesnakes

ObjectiveTo characterize the impact of mechanical positive pressure ventilation on heart rate (HR), arterial blood pressure, blood gases, lactate, glucose, sodium, potassium and calcium concentrations in rattlesnakes during anesthesia and the subsequent recovery period.Study designProspective, randomized trial.AnimalsTwenty one fasted adult South American rattlesnakes (Crotalus durissus terrificus).MethodsSnakes were anesthetized with propofol (15 mg kg⁻¹) intravenously, endotracheally intubated and assigned to one of four ventilation regimens: Spontaneous ventilation, or mechanical ventilation at a tidal volume of 30 mL kg⁻¹ at 1 breath every 90 seconds, 5 breaths minute⁻¹, or 15 breaths minute⁻¹. Arterial blood was collected from indwelling catheters at 30, 40, and 60 minutes and 2, 6, and 24 hours following induction of anesthesia and analyzed for pH, PaO₂, PaCO₂, and selected variables. Mean arterial blood pressure (MAP) and HR were recorded at 30, 40, 60 minutes and 24 hours.ResultsSpontaneous ventilation and 1 breath every 90 seconds resulted in a mild hypercapnia (PaCO₂ 22.4 ± 4.3 mmHg [3.0 ± 0.6 kPa] and 24.5 ± 1.6 mmHg [3.3 ± 0.2 kPa], respectively), 5 breaths minute⁻¹ resulted in normocapnia (14.2 ± 2.7 mmHg [1.9 ± 0.4 kPa]), while 15 breaths minute⁻¹ caused marked hypocapnia (8.2 ± 2.5 mmHg [1.1 ± 0.3 kPa]). Following recovery, blood gases of the four groups were similar from 2 hours. Anesthesia, independent of ventilation was associated with significantly elevated glucose, lactate and potassium concentrations compared to values at 24 hours (p < 0.0001). MAP increased significantly with increasing ventilation frequency (p < 0.001). HR did not vary among regimens.Conclusions and clinical relevanceMechanical ventilation had a profound impact on blood gases and blood pressure. The results support the use of mechanical ventilation with a frequency of 1–2 breaths minute⁻¹ at a tidal volume of 30 mL kg⁻¹ during anesthesia in fasted snakes.     

转基因植物对环境生态平衡的影响

根据近年来国内外关于转基因植物的研究进展,结合维持生态平衡的3个主要因素,即物种组成、环境因素及信息系统,论述转基因植物对环境生态平衡的影响.     

非洲菊切花保鲜剂的研究

本文研究由蔗糖、硝酸银、8-HQ(8-羟基喹啉)配制的不同保鲜剂对非洲菊切花插瓶寿命、水分平衡、鲜重变化及观赏值等指标的影响。结果表明：4％蔗糖 200mg／L硝酸银 200mg／L硫酸铝 320mg／L8-HQ的保鲜效果最好。     

土壤-植被系统演变对生物防沙工程的影响

包兰铁路横穿腾格里沙漠的沙坡头段 ,全长近 5 0km ,这里年降水量 186mm ,流动沙丘相对高度 2 0m以上 ,以 4m·a- 1 的速度向东南方向移动。无灌溉条件下、工程措施与生物措施相结合的防沙固沙工程体系始建于195 6年 ,近半个世纪以来该体系确保了包兰铁路的畅通无阻 ,累计经济效益逾百亿人民币。虽然当初的建设者充分考虑了耐旱的乡土树种、有效的工程固沙系统 ,但很难想到几十年后的土壤 -植被系统的演变会危及整个防护体系的稳定性。植被在从人工系统向自然系统的演变中经历了覆盖度从增加到减少的自疏过程、从灌木到半灌木到草本的变化 ,其降低风速和防风的功能减弱 ;土壤从流动风沙土发育成钙积旱成土 ,地表生物结皮发育 ,增加了表土的抗风蚀的能力 ;土壤 -植被系统的演变使得降水在生态系统中的再分配过程已经彻底改变 ,并由于防护林体系配置不完善 ,在 5 0～ 5 0 0cm的土层深度一个生物成因的干旱层逐渐形成 ,新的水分平衡格局明显威胁到现有的防风固沙体系。     

Effects of nitrogen and phosphorus fertilization on the growth and nutrition of hybrid poplars on Vancouver Island

The effects of nitrogen (N) and phosphorus (P) rates, P source, and method of P application were tested on growth and nutrition of four clones of hybrid poplar (Populus trichocarpa Torr. & Gray×P. deltoides Bartr. ex Marsh or P. trichocarpa×P. maximowiczii A. Henry) in a plantation on Vancouver Island. Treatments were applied shortly after planting. Nitrogen (0, 500kgNha^–1) was supplied as ammonium sulfate (AS); half of the AS was added at the start of the second growing season. Phosphorus (0, 100, 200kgPha^–1) was supplied as triple super phosphate (TSP), rock phosphate (RP), or diammonium phosphate (DAP) and banded or broadcast. Over four growing seasons, stem volumes increased with AS and P additions. The effects of AS and P each were greatest when the other was also added. Volume was greater when P was applied at 100kgha^–1 than in the P control and did not significantly increase further at 200kgPha^–1. Phosphorus additions were more effective when added as TSP or DAP than when added as RP, but only in P100 and when banded. Banding increased volume when P was applied at 200kgha^–1, but increases were significant only for DAP fertilized trees. Roto-tilling associated with the banding treatment also increased volume when AS and P were not added. Rankings of clones with respect to stem volume varied with N supply and changed over time.     

民勤绿洲荒漠过渡区辐射特征与热量平衡规律研究

根据巴丹吉林沙漠与腾格里沙漠交汇处的民勤绿洲荒漠过渡区的长期连续实测资料 ,分析了该地区的辐射特征 ,研究了热量平衡各分量的变化特征及热量平衡规律。在绿洲荒漠过渡区 ,其太阳辐射、净辐射和反射辐射具有明显的日进程和季节变化规律 ;太阳辐射月总量最大值 (12 0 2 3 3kW·m- 2 )出现在 8月份 ,最小值(5 2 0 6 5kW·m- 2 )出现在 11月份 ;流动沙丘表面的反射率高于有植被覆盖的红柳和白刺灌丛沙丘表面的反射率 ,在植被生长季节更为明显 ,反射率日最大值分别为流动沙丘 (37 5 % ) >白刺沙丘 (2 9 7% ) >红柳沙丘 (2 7 9% ) ;流动沙丘表面接受的净辐射是植被覆盖区的 5 0 %～ 70 %左右。在绿洲荒漠过渡区生态系统的热量平衡中 ,占比例最大的是潜热通量 ,其次为感热通量和土壤热通量。随着荒漠化程度的加深 ,生态系统反射率增加 ,净辐射减少 ,而且热量平衡中感热通量和土壤热通量所占比例增加 ,而潜热通量比例减小。     

Nitrogen-fixation dynamics in a cut-and-carry silvopastoral system in the subhumid conditions of Guadeloupe, French Antilles

This paper summarizes several studies on N recycling in a tropical silvopastoral system for assessing the ability of the system to increase soil fertility and insure sustainability. We analyzed the N₂ fixation pattern of the woody legume component (Gliricidia sepium), estimated the recycling rate of the fixed N in the soil, and measured N outputs in tree pruning and cut grass (Dichanthium aristatum). With this information, we estimated the N balance of the silvopastoral system at the plot scale. The studies were conducted in an 11-year-old silvopastoral plot established by planting G. sepium cuttings at 0.3 m × 2 m spacing in natural grassland. The plot was managed as a cut-and-carry system where all the tree pruning residues (every 2-4 months) and cut grass (every 40-50 days) were removed and animals were excluded. No N fertilizer was applied. Dinitrogen fixation, as estimated by the ¹⁵N natural abundance method, ranged from 60-90% of the total N in aboveground tree biomass depending on season. On average, 76% of the N exports from the plot in tree pruning (194 kg [N] ha^–1 yr^–1) originated from N₂ fixation. Grass production averaged 13 Mg ha^–1 yr^–1 and N export in cut grass was 195 kg [N] ha^–1 yr^–1. The total N fixed by G. sepium, as estimated from the tree and grass N exports and the increase in soil N content, was about 555 kg [N] ha^–1 yr^–1. Carbon sequestration averaged 1.9 Mg [C] ha^–1 yr^–1 and soil organic N in the 0-0.2 m layer increased at a rate of 166 kg [N] ha^–1 yr^–1, corresponding to 30% of N₂ fixation by the tree. Nitrogen released in nodule turnover (10 kg [N] ha^–1 yr^–1) and litter decomposition (40 kg [N] ha^–1 yr^–1) contributed slightly to this increase, and most of the recycled N came from the turnover or the activity of other below-ground tree biomass than nodules. This revised version was published online in June 2006 with corrections to the Cover Date.     

砂基栽培中磷素淋失防治研究进展

砂基栽培中的磷素淋溶损失不仅造成肥料利用率降低、农业生产成本上升,还能引起地下和地表水体富营养化。在综合中外有关文献的基础上,阐述了砂基栽培磷素淋溶问题的提出、影响磷素淋溶的因子、防治方法,并对砂基栽培磷素淋溶及其防治作了展望。     

马尾松种源对磷肥的遗传反应及根际土壤营养差异

利用5a生马尾松种源与磷肥互作试验林，研究不同种源对磷肥的遗传反应式样以及在低(缺)磷环境下的根际营养差异。结果发现，广东高州和广西岑溪两种源对磷肥的反应显著，属于对磷肥敏感型种源。广东信宜和福建武平两种源对磷肥反应不敏感，属于耐低磷型或对磷肥不敏感型种源，而江西崇义种源显著的磷肥效应仅在造林后头2～3年显示。对于根际土的的化学性质，不同种源虽有较大差异，但根际土的有机质、全氮、水解氮和有效磷含量一般显著高于非根际土。不同种源根际对土壤中的磷都具有活化作用和富集作用，尤其是广东信宜和福建武平两种源对土壤磷的活化和富集作用最为强烈，这可初步解释这两个种源对磷肥不敏感、能适应低(缺)磷胁迫的遗传机制。马尾松不同种源根际土壤并未发生明显的酸化。     

A split-root apparatus for examining the effects of hydraulic lift by trees on the water status of neighbouring crops

We describe a split-root system for examining the effects of hydraulic lift by trees on crop growth. In this system, upper lateral tree roots were grown in a container set on the ground through which the taproot of the tree could penetrate into the moist soil below. The container, with a radius of 0.5 m and a height of 0.20 m, consisted of two compartments divided by a waterproof barrier. A markhamia tree (Markhamia lutea (Benth.) Schumann) and upland rice (Oryza sativa (L.)) plants were planted in one compartment, with only rice plants planted in the other compartment. Irrigation of the container was ceased at the start of the experiment. The stomatal conductance of the rice plants in the associated side, in which both trees and rice plants were grown, declined more rapidly during the first drying period than in the rice-only compartment, suggesting that there was competition for water between the tree and the crop plants. However, during the later drying period, the rice plants in the associated side were green and viable, while those in the rice-only side became desiccated. Rice roots were seen intermingling with tree roots, and the soil water content in the associated site tended to be higher than in the rice-only side. It is likely that hydraulic lift occurred in the associated side and that water that had been transferred to the surface roots was released into the soil, enabling the rice plants in this compartment to remain viable. This novel system is useful for examination of the effects of hydraulic lift by trees on the growth of neighbouring crops. This revised version was published online in June 2006 with corrections to the Cover Date.